Journal: Molecular Neurodegeneration

Article Title: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

doi: 10.1186/s13024-020-00409-0

Figure Lengend Snippet: Trem2 Y38C variant and Trem2 deficient mice affects microglial morphology. a CRISPR/Cas9 strategy to generate the tyrosine to cystine point mutation (red) in exon 2 of murine Trem2 to create the Trem2 Y38C/Y38C mouse model. The sequences for the reference genome, guide RNA, and homology directed repair (HDR), which indicates the Y38C variant (red) and a silent mutation (blue) to ablate the protospacer adjacent motif (PAM), are shown. b Quantification of Trem2 mRNA levels in cortical lysates showed similar Trem2 expression in WT (black) and Trem2 Y38C/Y38C (green) mice and no expression in Trem2 −/− (red) mice. Data are presented as mean ± SEM fold change normalized gene expression relative to WT. N = 8 (4 males and 4 females for each group) c Immunohistochemistry for TREM2 (green) and Iba-1 (red) is shown in cortices of 6 month WT, Trem2 Y38C/Y38C , and Trem2 −/− mice. Merged imaged indicates colocalization of TREM2 staining with Iba-1+ (red) cell bodies in WT and Trem2 Y38C/Y38C mice. No TREM2 staining was observed in Trem2 −/− mice. (representative images from females). d Quantification of total TREM2 in WT, Trem2 Y38C/Y38C , and Trem2 −/− cortical lysates by ELISA indicates slightly higher levels of TREM2 in Trem2 Y38C/Y38C as compared to the WT mice. e sTREM2 levels, quantified in TBS soluble cortical extracts, were similar in both Trem2 Y38C/Y38C and WT. f Quantification of the number of Iba-1 positive cells showed similar counts in cortices of 6 months old WT, Trem2 Y38C/Y38C and Trem2 −/− mice. g Quantification of Iba-1 immunoreactive area showed increased staining in Trem2 Y38C/Y38C and Trem2 −/− mice. f-g Data represented as mean ± SEM, N = 6 (3 males and 3 females for each group; n = 56 fields for WT, n = 89 fields for Trem2 Y38C/Y38C , and n = 112 fields for Trem2 −/− ). h Skeletonized reconstructions (representative reconstructions from females), i quantification of number of branches and j number of junctions in microglia from the cortices of 6 months old WT, Trem2 Y38C/Y38C and Trem2 −/− mice. h-j Data represented as mean ± SEM, N = 6 (3 males and 3 females for each group, each data point represents microglia analyzed). b,d-i One-way ANOVA with Tukey’s post hoc test. * P < 0.05, *** P < 0.001, **** P < 0.0001

Article Snippet: Wells were washed 4 times with 0.05% Tween in PBS and incubated with 0.25μg/ml of the TREM2 biotinylated detection antibody (R&D Systems, BAF1729) for 1 h at RT.

Techniques: Variant Assay, CRISPR, Mutagenesis, Expressing, Gene Expression, Immunohistochemistry, Staining, Enzyme-linked Immunosorbent Assay

Journal: Molecular Neurodegeneration

Article Title: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

doi: 10.1186/s13024-020-00409-0

Figure Lengend Snippet: Loss of functional TREM2 alters gene expression associated with oligodendrocyte/myelin and neuronal function. a Volcano plot showing differentially expressed genes in 6 month Trem2 Y38C/Y38C versus WT cortices and Trem2 −/− versus WT cortices. Dashed line represents P < 0.005. Significantly altered genes are shown in green with downregulated and upregulated genes above |logFC| > 0.58 threshold of in green. b-c Enrichr pathway analysis of the shared downregulated genes between Trem2 Y38C/Y38C and Trem2 −/− mice (ranked by P value; Fischer’s exact test, P-adjusted, Benjamini-Hochberg method for correction for multiple hypotheses testing). b Tissue enrichment analysis showing significant enrichment in brain regions. Dashed line represents P < 0.005. c Enrichment analysis for specific cellular compartments showing myelin-related enrichment. Dashed line represents P < 0.005. d Top 10 pathways significantly altered using IPA pathway analysis. Analysis based on significantly differentially expressed genes between Trem2 Y38C/Y38C versus WT mice and Trem2 −/− vs WT mice. Yellow line represents P < 0.05 yellow line (ranked by P value, Fischer’s extact test). e Top 5 predicted upstream regulators of differentially expressed genes in Trem2 Y38C/Y38C versus WT mice and Trem2 −/− vs WT mice using IPA Upstream Regulator analysis. Activated (black) and inhibited (grey) upstream regulators are shown. P value, Fisher’s exact test. Sample sizes: WT mice, N = 8 (4 males, 4 females); Trem2 Y38C/Y38C mice, N = 8 (4 males, 4 females); Trem2 −/− mice, N = 5 (1 male, 4 females)

Article Snippet: Wells were washed 4 times with 0.05% Tween in PBS and incubated with 0.25μg/ml of the TREM2 biotinylated detection antibody (R&D Systems, BAF1729) for 1 h at RT.

Techniques: Functional Assay, Gene Expression

Journal: Molecular Neurodegeneration

Article Title: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

doi: 10.1186/s13024-020-00409-0

Figure Lengend Snippet: Oligodendrocyte/Myelin impairments in adult Trem2 Y38C/Y38C and Trem2 −/− mice. a-c Mbp, Mobp and Olig2 transcript levels were determined by quantitative-PCR. a Mbp transcripts were reduced in both Trem2 Y38C/Y38C and Trem2 −/− cortical lysates and b-c Mobp and Olig2 mRNA reduced significantly in Trem2 −/− cortical lysates. d Western blot analysis and e Quantification of myelin proteins CNPase and MBP. CNPase levels were reduced significantly in Trem2 −/− mice and Mbp levels were significantly reduced in both Trem2 Y38C/Y38C and Trem2 −/− mice. a-e Data represented as mean ± SEM. N = 4 (2 males, 2 females); One-way ANOVA with Tukey’s post hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Wells were washed 4 times with 0.05% Tween in PBS and incubated with 0.25μg/ml of the TREM2 biotinylated detection antibody (R&D Systems, BAF1729) for 1 h at RT.

Techniques: Real-time Polymerase Chain Reaction, Western Blot

Journal: Molecular Neurodegeneration

Article Title: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

doi: 10.1186/s13024-020-00409-0

Figure Lengend Snippet: Decreased synaptic elements in adult Trem2 Y38C/Y38C and Trem2 −/− mice. Microdissected a,b cortices and c,d hippocampi of WT, Trem2 Y38C/Y38C and Trem2 −/− mice were analyzed using western blot with antibodies against PSD95 and synaptophysin. a,b Trem2 Y38C/Y38C and Trem2 −/− mice showed significant reduction in synaptophysin but not in PSD-95 protein levels. c,d PSD95 and synaptophysin protein levels were significantly reduced in hippocampi of Trem2 Y38C/Y38C and Trem2 −/− mice. e Comparison of PSD95 and synaptophysin protein expression in hippocampal lysates of WT, Trem2 Y38C/Y38C and Trem2 −/− mice at P20 and 6 months. Comparing P20 to 6 months, PSD95 levels increase significantly (black asterisk) in WT mice (black), while both Trem2 Y38C/Y38C (green) and Trem2 −/− (red) mice show comparatively less increase resulting in reduced PSD95 levels at 6 months as compared to WT. Synaptophysin levels were maintained in WT mice (black) and Trem2 Y38C/Y38C (green), while Trem2 −/− (red) mice showed significantly lower levels at 6 months as compared to P20 (red asterisk) indicating loss of presynaptic elements in adult mice. Sample sizes for each group: N = 6–8 (3–4 each, males and females). Data are shown as mean ± SEM. b,d One-way ANOVA with Tukey’s post hoc test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. e Two-way ANOVA with Bonferroni’s post hoc test; * P < 0.05, WT mice at 6 months versus P20 (black) and Trem2 −/− mice at 6 months versus P20 (red)

Article Snippet: Wells were washed 4 times with 0.05% Tween in PBS and incubated with 0.25μg/ml of the TREM2 biotinylated detection antibody (R&D Systems, BAF1729) for 1 h at RT.

Techniques: Western Blot, Comparison, Expressing

Journal: Molecular Neurodegeneration

Article Title: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

doi: 10.1186/s13024-020-00409-0

Figure Lengend Snippet: Hippocampal synaptic plasticity is reduced in Trem2 Y38C/Y38C and Trem2 −/− mice. a Input-output curves were similar in Trem2 Y38C/Y38C , Trem2 −/− , and WT mice. b,c Hippocampal Schaffer collateral-CA1 LTP is significantly reduced in Trem2 Y38C/Y38C (green) and Trem2 −/− (red) mice as compared to WT mice (black) at 6 months of age. b Superimposed representative traces before (black) and 60 min after high-frequency stimulation (gray) in each genotype. c Mean time course of fEPSP slope in hippocampal slices from WT (black), Trem2 Y38C/Y38C (green) and Trem2 −/− (red). Arrow indicates time of high frequency stimulation. d Average of normalized fEPSP slope for final 10 min of recording (60–70 min) relative to 10-min baseline average (dotted line). Data are shown as mean ± SEM. Sample sizes: WT mice, N = 15 (7 females and 8 males, n = 33 recordings); Trem2 Y38C/Y38C mice, N = 7 (3 females and 4 males, n = 20 recordings); Trem2 −/− mice, N = 8 (4 males and 4 females, n = 15 recordings). One-way ANOVA with Tukey’s post hoc test. *** P < 0.001, **** P < 0.0001

Article Snippet: Wells were washed 4 times with 0.05% Tween in PBS and incubated with 0.25μg/ml of the TREM2 biotinylated detection antibody (R&D Systems, BAF1729) for 1 h at RT.

Techniques:

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Flow cytometry analysis of TREM2 (open tracings) expression on CD11b+ cells derived from the cortex of normal and EAE-diseased mice at day 4 after onset of clinical symptoms as well as from EAE-diseased mice at day 18 after onset of clinical symptoms. Isotype controls are shown in grey-filled tracings. TREM2 expression is detected on CD11b+ microglia/macrophages in the cortex of EAE-diseased mice. The percentages of TREM2+ cells are indicated in the upper-right corner of each histogram. Selected representative histograms are shown. (B) Quantitative analysis of TREM2 expression on microglia by flow cytometry gated for CD11b+ cells. TREM2 expression is detected only on a minority of microglia and perivascular macrophages in the normal CNS tissue, but is up-regulated and detected in the spinal cord on the majority of CD11b+ cells at day 4 after onset of clinical symptoms of EAE. Data are presented as mean ± SEM. Tissues were derived from four EAE and four normal mice.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Flow Cytometry, Expressing, Derivative Assay

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Flow cytometry analyses of BM-MC. Bone marrow cells were cultured for 10 d in GM-CSF–containing medium and then analyzed by flow cytometry with specific antibodies (open tracings) directed against CD45, CD11b, CD11c, MHC class II, CD80, CD86, F4/80, CD36, TREM2, Sca1, c-kit, and CD133. Cells showed expression of CD45, F4/80, CD11b, CD11c, and CD36. The percentage of positive cells is indicated in the upper-right corner of each histogram. Isotype controls are shown in grey-filled tracings. (B) Lentiviral vector design. To express TREM2 in BM-MC, the mouse TREM2 coding sequence was cloned under the CMV promoter followed by a second CMV promoter and GFP (plenti TREM2). The same vector without TREM2 was used as a control (plenti GFP). (C) Gene transcripts in myeloid cells transduced with TREM2 vector (TREM2-BM-MC) or control GFP vector (GFP-BM-MC) for TREM2, DAP12, and the housekeeping gene GAPDH were analyzed by RT-PCR. Gene transcripts for TREM2 were strongly detected in TREM2-BM-MC and weakly in GFP-BM-MC. Both TREM2-transduced myeloid cells and control GFP vector–transduced myeloid cells showed gene transcription for DAP12, the TREM2 adapter and signaling molecule. (D) Flow cytometry analysis of TREM2-transduced BM-MC (bold line) and GFP-transduced BM-MC (narrow line), both labeled with TREM2-specific antibodies. Isotype control antibody is shown in filled grey. Cell surface expression of TREM2 was detected on myeloid cells after lentiviral transduction with TREM2, but not on control GFP vector–transduced myeloid cells.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Flow Cytometry, Cell Culture, Expressing, Plasmid Preparation, Sequencing, Clone Assay, Transduction, Reverse Transcription Polymerase Chain Reaction, Labeling

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) TREM2-transduced myeloid cells showed increased phagocytosis of beads and of apoptotic neuron–enriched cultures. Analysis of the phagocytosis of beads (left graph), TREM2-transduced myeloid cells (open bars), control GFP vector–transduced myeloid cells (filled bars), or cultured monocytes (grey bars) after stimulation with TREM2-specific antibodies either untreated (−) or treated with ERK inhibitor (+). Phagocytosis assay (right graph) of apoptotic cells derived from neuron-enriched cultures by TREM2-transduced myeloid cells (open bars), control GFP vector–transduced myeloid cells (filled bars), or cultured monocytes (grey bars), either treated with ERK inhibitor (+) or untreated (−). Transduction of myeloid cells (BM-MC) with TREM2 increased the phagocytosis of beads and apoptotic cells that were dependent on ERK phosphorylation. Data are presented as mean ± SEM. Unpaired t -test: p < 0.001 (TREM2 versus GFP) for BM-MC without ERK inhibitor and with bead phagocytosis, and p < 0.001 (TREM2 versus GFP) for BM-MC without ERK inhibitor and with phagocytosis of apoptotic neurons. Five independent experiments were performed, and each of them was done in quadruplicate. (B) Anti-inflammatory activity of TREM2 stimulation. Myeloid cells were transduced with TREM2 (open bars) or control GFP vector (filled bars). Myeloid cells were cultured on plates coated with TREM2 cross-linking antibodies (left graph). One hour later, cells were stimulated with LPS. Gene transcript levels for TNFα, IL-1β, NOS2, and IL-10 were quantified by real-time PCR after 48 h. TREM2-transduced myeloid cells showed reduced gene transcript levels of IL-1β and NOS2 and an increased level of IL-10. In addition, apoptotic cells derived from neuron-enriched cultures were added to myeloid cells (right graph). Gene transcript levels for TNFα, IL-1β, NOS2, and IL-10 were quantified by real-time PCR after 48 h. Data are presented as mean ± SEM. Unpaired t -test: p < 0.001 (TREM2 versus GFP) with TREM2 plus LPS for IL-1, p = 0.0205 (TREM2 versus GFP) with TREM2 plus LPS for IL-10, p = 0.0149 (TREM2 versus GFP) with coculture for IL-1, and p = 0.012 (TREM2 versus GFP) with coculture for NOS2. Four independent experiments were performed. (C) Phosphorylation of ERK after stimulation of TREM2. Myeloid cells were transduced with TREM2 and cultured for 1 h on plates coated with TREM2 cross-linking antibodies (+) or isotype control antibodies (−). Cross-linking of TREM2 increased the amount of phosphorylated ERK (phospho ERK) in relation to total ERK (ERK). (D) TREM2-transduced myeloid cells showed an increased release of IL-10 after stimulation of TREM2 plus LPS or coculture with apoptotic neuron–enriched cultures. Myeloid cells were transduced with TREM2 (open bars) or with control GFP vector (filled bars), cultured on plates coated with TREM2 cross-linking antibodies, and stimulated with LPS 1 h later or supplemented with apoptotic cells derived from neuron-enriched cultures. The amount of IL-10 released in the supernatant was determined by ELISA. Data are presented as mean ± SEM. Unpaired t -test: p = 0.044 (TREM2 versus GFP) for TREM2 plus LPS, and p = 0.0054 (TREM2 versus GFP) for coculture. Four independent experiments were performed.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Plasmid Preparation, Cell Culture, Phagocytosis Assay, Derivative Assay, Transduction, Activity Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Detection of invaded myeloid cells in the spinal cord of EAE-diseased mice. Histological sections were obtained at day 2 after intravenous injection of GFP-transduced cells in EAE-diseased mice. Myeloid cells were injected at day 4 after first clinical signs of EAE. Sections were labeled with CD45-specific antibodies. Injected myeloid cells expressed CD45 and accumulated in inflammatory lesions. Scale bar indicates 50 μm. (B) No Iba1 antigen was detected on invaded myeloid cells by double immunofluorescence labeling. GFP+ cells were analyzed in the spinal cord of EAE-diseased mice 4 d after intravenous injection of GFP-transduced myeloid cells. Myeloid cells were applied 4 d after first clinical signs of the disease. Invaded cells showed round appearance, reminiscent of activated macrophage/microglial cells. Scale bar indicates 10 μm. (C) Quantification of cells invaded into the spinal cord of EAE-diseased mice. TREM2-transduced BM-MC or GFP-transduced BM-MC were injected intravenously at day 4 after the first clinical signs of the disease appeared. The BM-MCs migrated into spinal cord lesions within 2 h after intravenous application and remained there for 2–4 d. No GFP+ cells were detected within the spinal cord at day 7 after intravenous application. Data are presented as mean ± SEM. (D) Flow cytometry analysis of TREM2 expression on TREM2-transduced and GFP-transduced myeloid cells obtained from the spinal cord of EAE-diseased mice at day 2 after application. Expression of TREM2 was detected on myeloid cells transduced with TREM2 (red tracing), but not on cells transduced with GFP (green tracing). Tracings shown are from cells gated for GFP. Control antibody isotype is shown as grey-filled tracing. (E) Flow cytometry analysis of CD45 expression on GFP-transduced myeloid cells before injection (green tracing) and on GFP+ cells invaded into the spinal cord (red tracing) at day 4 after injection. Engrafted myeloid cells showed reduced levels of CD45 expression, reminiscent of microglia. Tracings shown are from cells gated for GFP. Control antibody isotype is shown as grey-filled tracing.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Injection, Labeling, Immunofluorescence, Flow Cytometry, Expressing, Transduction

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Immunostaining of macrophages (MAC3), T cells (CD3), degenerated MBP (dMBP), and Lamp2 in the spinal cord of EAE-diseased mice at day 2 after injection of TREM2-transduced myeloid cells (TREM-BM-MC), GFP-transduced myeloid cells (GFP-BM-MC), or vehicle control (PBS). Cells were injected intravenously in EAE-diseased mice at day 4 after first clinical signs of the disease. Scale bars for MAC3 and CD3 indicate 300 μm. Scale bars for dMBP and Lamp2 indicate 50 μm. (B) Quantification of the number of MAC3- and CD3+ cells, degenerative MBP, and LAMP2-positive cells. EAE-diseased mice were injected at day 4 after first clinical signs of the disease with TREM2-transduced myeloid cells (TREM2-BM-MC), GFP-transduced myeloid cells (GFP-BM-MC), or vehicle control (PBS). At day 2 after cell injection, spinal cords were removed, stained, and analyzed. Macrophages were labeled by MAC3 and T cells by CD3-specific antibodies. Cell therapy by myeloid precursors did not affect the spinal cord immune cell infiltrates. Furthermore, quantification of cells stained for degenerated MBP (dMBP) in the spinal cord of EAE-diseased mice at day 2 after injection was performed, and the amount of degenerated MBP was reduced by cell therapy with TREM2-transduced myeloid cells. Quantification of Lamp2-positive cells (LAMP2) in spinal cord lesions of EAE-diseased mice at day 2 after cell injection was performed. The number of Lamp2-positive cells showing phagocytic activity was increased by the intravenous injection of TREM2-BM-MC. For quantitative analysis, three lumbar spinal cord sections of three mice per group were examined. Data are presented as mean ± SEM. ANOVA, followed by unpaired t -test: p = 0.0422 (TREM2 versus GFP) for dMBP, and p = 0.0069 (TREM2 versus GFP) for LAMP2. (C) Anti-inflammatory cytokine milieu in the spinal cord after treatment with TREM2-transduced myeloid cells. Real-time RT-PCR of cytokines and growth factors at day 6 after first clinical signs of the disease and 2 d after injection of TREM2-transduced myeloid cells ( n = 3), control GFP vector–transduced myeloid cells ( n = 3), or PBS as vehicle control ( n = 3). Relative gene transcript levels of TNFα, IFNγ, and IL-1β were decreased. ANOVA followed by unpaired t -test: p = 0.0324 (TREM2 versus GFP) for TNFα, p = 0.0111 (TREM2 versus PBS) for TNFα, p = 0.0068 (TREM2 versus PBS) for IFNγ, and p = 0.0215 (TREM2 versus PBS) for IL-1β. Each PCR experiment was performed in quadruplicate.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Immunostaining, Injection, Staining, Labeling, Activity Assay, Quantitative RT-PCR, Plasmid Preparation

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Treatment of EAE-diseased mice by TREM2-transduced myeloid cells at the peak of the disease. Clinical EAE score of mice injected at day 4 after first clinical signs of the disease (arrow) with TREM2-transduced myeloid cells (black tracing), control GFP vector–transduced myeloid cells (green tracing), or PBS (red tracing). Intravenous application of 5 × 10 6 TREM2-transduced myeloid cells ameliorated the clinical score of EAE. Data are presented as mean ± SD. Mice per group: n = 6 or n = 7. ANOVA followed by unpaired t -test: p = 0.0015 (TREM2 versus GFP) and p = 0.0063 (TREM2 versus PBS) at day 20; p = 0.0045 (TREM2 versus GFP) and p = 0.0046 (TREM2 versus PBS) at day 30. (B) TREM2-transduced myeloid cells injected at the onset of the disease do not affect EAE. Clinical EAE score of mice injected 1 d after the first clinical signs of the disease (arrow) with 5 × 10 6 TREM2-transduced myeloid cells (black), 5 × 10 6 control GFP vector–transduced myeloid cells (green), or PBS (red). No effect on the clinical EAE score was observed. Data are presented as mean ± SD. Mice per group: n = 3. (C) Treatment of EAE by TREM2-transduced myeloid cells. Clinical EAE score of mice injected at day 4 after first clinical signs of the disease with 2 × 10 6 TREM2-transduced myeloid cells (black), 5 × 10 6 TREM2-transduced myeloid cells (grey), or PBS control (red). Intravenous application of 2 × 10 6 or 5 × 10 6 TREM2-transduced myeloid cells ameliorated the clinical score of EAE. Data are presented as mean ± SD. Mice per group: n = 3. ANOVA followed by unpaired t -test: p = 0.0023 (2 × 10 6 cells versus PBS) and p = 0.0068 (5 × 10 6 cells versus PBS) at day 20; p = 0.0107 (2 × 10 6 cells versus PBS) at day 30.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Injection, Plasmid Preparation

Journal: PLoS Medicine

Article Title: TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

doi: 10.1371/journal.pmed.0040124

Figure Lengend Snippet: (A) Reduced spinal cord axonal injury and decreased demyelination after cell therapy by TREM2-transduced myeloid cells. Axonal injury and damage were analyzed by immunostaining with specific antibodies directed against APP and dephosphorylated neurofilament (SMI32) at day 14 after cell application. Demyelination was determined by LFB staining at day 14 after cell application. Mice were injected at day 4 after first clinical signs of EAE by TREM2-transduced myeloid precursor cells (TREM2-BM-MC), GFP-transduced BM-MC (GFP-BM-MC), or PBS control. Representative histological pictures are shown. Scale bars for APP and LFB indicate 200 μm, and for inserts indicate 50 μm. Scale bar for SMI32 indicates 50 μm, and for insert indicates 20 μm. (B) Quantification of axonal damage and demyelination 14 d after cell application in EAE with TREM2-transduced myeloid cells (black bars), control GFP vector–transduced myeloid cells (green bars), or vehicle PBS control (red bars). Level of axonal injury (APP-positive deposits/mm 2 ), axonal damage (relative number of SMI32-positive axons), and demyelination (loss of LFB, as a percentage) were significantly reduced after cell therapy with TREM2-transduced BM-MC. For quantitative analysis, at least three lumbar spinal cord sections of three mice per group were investigated. Data are presented as mean ± SEM. ANOVA followed by unpaired t -test: p = 0.0425 (TREM2 versus GFP) and p = 0.0759 (TREM2 versus PBS) in APP; p = 0.0012 (TREM2 versus GFP) and p = 0.0098 (TREM2 versus PBS) in SMI32; p = 0.0465 (TREM2 versus GFP) and p = 0.00450 (TREM2 versus PBS) in LFB.

Article Snippet: Furthermore, cells were stained with anti-TREM2 monoclonal antibodies (R&D Systems) followed by biotin-conjugated anti-rabbit IgG (Dianova) and CyChrome-conjugated streptavidin (BD Biosciences Pharmingen).

Techniques: Immunostaining, Staining, Injection, Plasmid Preparation